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05 / 05   Optical instruments

Application note

Microscope and Telescope

Distinguish image enlargement from angular magnification in two-lens instruments.

A microscope and a telescope combine an objective with an eyepiece, but their input geometry differs. The microscope examines a nearby object; the telescope typically receives nearly parallel light from a distant scene. Following the intermediate image explains why their magnification formulas differ and which distances must be controlled.

A microscope’s intermediate image

Consider two positive thin lenses in air, separated by LL. In a finite-conjugate microscope, the objective forms a real intermediate image. For relaxed viewing, that image sits at the eyepiece’s front focal plane, so light leaves the eyepiece parallel. If their focal lengths are fof_o and fef_e:

vo=L−fe,uo=fovovo−fo,mo=−vouov_o=L-f_e,\qquad u_o=\frac{f_o v_o}{v_o-f_o},\qquad m_o=-\frac{v_o}{u_o}

A finite-conjugate microscope objective produces a real inverted intermediate image at the eyepiece focal plane; each point then leaves as a parallel bundle.

View diagram at full size

The eyepiece acts as a magnifier, with relaxed-view angular gain D/feD/f_e relative to viewing the intermediate image unaided at distance DD. Comparing the complete instrument with the original specimen viewed unaided at DD gives:

M=moDfeM=m_o\frac{D}{f_e}

Unlike mom_o, which compares physical image heights, MM compares apparent angles. The conventional reference D=250D=250 mm is a comparison distance, not an assumed capability of every eye. All distances must use the same units.

Derivation: microscope magnification combines image scale and viewing angle

Let the specimen height be hh and the intermediate-image height be hi=mohh_i=m_oh. The objective’s finite image has transverse magnification mo=−vo/uom_o=-v_o/u_o. For relaxed viewing, the intermediate image is one eyepiece focal length in front of the eyepiece, so the lens separation satisfies:

L=vo+fe,vo=L−feL=v_o+f_e,\qquad v_o=L-f_e

Applying the objective’s thin-lens equation gives:

1fo=1uo+1vo⟹uo=fovovo−fo\frac{1}{f_o}=\frac{1}{u_o}+\frac{1}{v_o} \qquad\Longrightarrow\qquad u_o=\frac{f_ov_o}{v_o-f_o}

Use consistent signed apparent angles for the specimen and its viewed image. In the paraxial approximation, the eyepiece presents an apparent angle αoptics≈hi/fe\alpha_{\mathrm{optics}}\approx h_i/f_e. The unaided specimen at reference distance DD subtends αreference≈h/D\alpha_{\mathrm{reference}}\approx h/D. Thus:

M=αopticsαreference=hi/feh/D=moDfeM=\frac{\alpha_{\mathrm{optics}}}{\alpha_{\mathrm{reference}}} =\frac{h_i/f_e}{h/D}=m_o\frac{D}{f_e}

The objective contributes a physical image-height ratio; the eyepiece contributes the angular factor D/feD/f_e. Multiplying two finite-image transverse magnifications would describe a different output condition. A camera placed at the intermediate image records the objective’s image scale and does not automatically acquire the eyepiece’s visual angular factor.

For fo=10f_o=10 mm, fe=25f_e=25 mm and L=135L=135 mm, the objective image is at 110 mm and the object is at 11 mm. Thus mo=−10m_o=-10 and M=−100M=-100: the view is inverted. A real intermediate image requires L>fo+feL>f_o+f_e; enlargement requires L>2fo+feL>2f_o+f_e. Infinity-corrected microscopes use a tube lens and need their own optical model.

An afocal telescope

A Keplerian telescope uses two positive lenses. For a distant scene and relaxed viewing, the objective’s rear focal plane coincides with the eyepiece’s front focal plane:

L=fo+fe,M=−fofeL=f_o+f_e,\qquad M=-\frac{f_o}{f_e}

In an afocal Keplerian telescope the shared focal plane connects two collimated bundles; the eyepiece images the objective aperture into an exit pupil.

View diagram at full size

For a distant object with small full angular extent α\alpha in radians, its intermediate-image size is:

Hi≈foαH_i\approx f_o\alpha

That real image lies at the objective’s rear focal plane. The infinity-input model specifies angular extent rather than a finite object height and distance, so an ordinary objective transverse magnification is not reported.

Derivation: telescope angular magnification and intermediate-image size

A distant object supplies a parallel ray bundle for each field point. Let qinq_{\mathrm{in}} be the signed paraxial propagation angle of one bundle. The objective forms its image at height hi≈foqinh_i\approx f_oq_{\mathrm{in}} in the rear focal plane. With that plane at the eyepiece’s front focus, the outgoing angle is:

qout≈−hife⟹qoutqin=−fofeq_{\mathrm{out}}\approx-\frac{h_i}{f_e} \qquad\Longrightarrow\qquad \frac{q_{\mathrm{out}}}{q_{\mathrm{in}}}=-\frac{f_o}{f_e}

Expressing both angles as viewing directions reverses both signs, leaving their ratio unchanged. Hence visual angular magnification is M=−fo/feM=-f_o/f_e. The negative sign denotes inversion.

A centered object spanning full angle α\alpha has edges at ±α/2\pm\alpha/2. Their image-height separation is approximately:

Hi≈foα2−(−foα2)=foαH_i\approx f_o\frac{\alpha}{2}-\left(-f_o\frac{\alpha}{2}\right)=f_o\alpha

Here HiH_i is a positive full size, not a signed image height. Convert degrees to radians before calculating: αrad=αdegπ/180\alpha_{\mathrm{rad}}=\alpha_{\mathrm{deg}}\pi/180. This is a paraxial field estimate, not a wide-angle distortion model. A final afocal output has an angular image; the intermediate real image still has a definite physical size.

Exit pupil size and position

The exit pupil is the eyepiece’s image of the aperture stop. If the objective aperture is the stop and downstream optics do not clip the rays, its diameter DobjectiveD_{\mathrm{objective}} gives:

DexitDobjective=fefo=1∣M∣\frac{D_{\mathrm{exit}}}{D_{\mathrm{objective}}}=\frac{f_e}{f_o} =\frac{1}{|M|}

Both DD quantities here are aperture diameters, distinct from the microscope’s reference viewing distance. Pupil size describes light delivery to the observer rather than scene-image size or resolution.

Derivation: the eyepiece images the objective aperture into the exit pupil

For pupil imaging, the objective aperture is the eyepiece’s object. Its distance from the eyepiece is spupil=L=fo+fes_{\mathrm{pupil}}=L=f_o+f_e. Apply the thin-lens equation to this aperture image:

1fe=1L+1vpupil⟹vpupil=feLL−fe=fe(1+fefo)\frac{1}{f_e}=\frac{1}{L}+\frac{1}{v_{\mathrm{pupil}}} \qquad\Longrightarrow\qquad v_{\mathrm{pupil}}=\frac{f_eL}{L-f_e} =f_e\left(1+\frac{f_e}{f_o}\right)

This positive distance locates the ideal exit-pupil plane downstream from the eyepiece. The signed transverse magnification of the pupil image is:

mpupil=−vpupilL=−fefom_{\mathrm{pupil}}=-\frac{v_{\mathrm{pupil}}}{L} =-\frac{f_e}{f_o}

A diameter is positive, so Dexit=∣mpupil∣DobjectiveD_{\mathrm{exit}}=|m_{\mathrm{pupil}}|D_{\mathrm{objective}}. Combining this with ∣M∣=fo/fe|M|=f_o/f_e gives the reciprocal diameter ratio. This is magnification of the aperture image, separate from magnification of the scene. Real eyepiece assemblies require their actual pupil and principal-plane data to establish usable eye position.

With fo=200f_o=200 mm and fe=25f_e=25 mm, the separation is 225 mm and M=−8M=-8. An object spanning 0.5∘0.5^\circ forms an intermediate image approximately 1.75 mm across. A 40 mm objective gives a 5 mm exit pupil, located 28.125 mm beyond the ideal eyepiece plane under the stated stop assumption.

Greater apparent size does not guarantee additional detail. Objective aperture, wavelength, aberrations, field coverage, and pupil access still constrain useful performance. For camera-based measurement, specify the sensor-plane image scale separately from the visual angular magnification quoted for an eyepiece.

Explore the model

Finite-conjugate microscope

Calculate objective imaging and relaxed-eye angular magnification for two positive lenses.

Change the example values to update the results and diagram. Use the stated units and sign convention.

Must exceed objective plus eyepiece focal length.

Comparison distance for the unaided eye; not an individual near-point measurement.

Object distance before objective
11mm
Intermediate image after objective
110mm
Objective transverse magnification
-10×
Signed total angular magnification
-100×

The objective forms an inverted enlarged intermediate image. Final viewing is relaxed.

Finite-conjugate microscope: updated diagramObjective forms an image 110 mm downstream, one eyepiece focal length before the eyepiece. Output is collimated for relaxed viewing.ObjectiveEyepieceObjectIntermediate images = 11 mm · s′obj = 110 mm
Input-responsive schematic; not to scale. Scroll horizontally on narrow screens. Positions illustrate the calculated conjugates; this is not a full ray trace.
Formula & assumptions

s′obj = L − fe. sobj = fo s′obj/(s′obj − fo). mobj = −s′obj/sobj. Angular M = mobj D/fe.

Two positive thin lenses in air. The objective image lies at the eyepiece front focal plane, so final rays are collimated for relaxed viewing. Magnification is signed relative to unaided viewing at D. This finite-conjugate geometry is not an infinity-corrected microscope or the approximate tube-length rule.

Explore the model

Keplerian telescope

Relate a distant angular object to its intermediate image and the afocal viewing system.

Change the example values to update the results and diagram. Use the stated units and sign convention.

Full extent, not half-angle. Calculation range 0–5°; 0 represents an on-axis point.

Signed angular magnification
-8×
Afocal lens separation
225mm
Intermediate plane after objective
200mm
Intermediate image full size, H
1.74533mm
Objective transverse magnification
Not defined for an object at infinity
Exit-pupil diameter
5mm
Exit pupil after eyepiece
28.125mm

The angular input determines full intermediate image size. It does not introduce a finite object distance or change afocal spacing.

Keplerian telescope: updated diagramColored rays show the on-axis pupil bundle at afocal spacing. The dark vertical marker separately shows full field-image extent 1.74533 mm at 200 mm after the objective, from full angular object extent 0.5 degrees. It is not an off-axis ray trace. Signed angular magnification -8 and exit pupil 5 mm assume an objective aperture stop.ObjectiveEyepieceFull field image H = 1.74533 mmColored rays: on-axis pupil bundle
Input-responsive schematic; not to scale. Scroll horizontally on narrow screens. The numerical results define the distances and angles.
Formula & assumptions

Angular M = −fo/fe. Lens separation = fo + fe. Intermediate plane = fo after the objective; full image size H ≈ fo αrad, where αrad = αdeg π/180. Exit-pupil diameter = objective diameter / |M|. Pupil distance after eyepiece = fe(1 + fe/fo).

Positive thin lenses in air, distant object, and relaxed-eye afocal spacing. Both incident and outgoing angles must remain small; the 5° input range is not a lens-accuracy guarantee. Objective transverse magnification is undefined for an object at infinity. Negative angular M denotes inversion. Pupil results assume the objective is the aperture stop and the eyepiece does not vignette the beam.

From estimate to application

Evaluate the complete optical arrangement.

Magnification is one requirement. Field, resolution, pupil access, and mechanical spacing determine whether the arrangement fits the application.

Discuss your optical system